Abnormal Human Serum Globulins

Action of papain on human serum globulins.

Studies on the degradation of animal and human serum antibody type proteins by various proteolytic enzymes have indicated that molecules of approximately one-half and one-quarter the size of the parent 160,000-molecular weight protein may be readily formed (l-5). Porter (6) has recently shown that rabbit y-globulin is degraded by papain into fragments of the above size range and has separated s...

Separation and Determination of the Total Globulins of Human Serum.

A method for isolation and determination of total globulins of human serum, carried out in a single tube without transfer, is described. Serum is treated with 2% (w/v) TCA in ethanol at room temperature to precipitate the globulins quantitatively as a gelatinous white precipitate easily recovered by centrifugation. The supernatant fluid is discarded and the precipitate washed with 2% TCA in eth...

The Alleged Transformation of Serum Albumin into Serum Globulins.

An automated estimation of serum globulins.

UTOMATIC ESTIMATION of serum globulins in human serum is normally measured as the difference between the total protein and the albumin as estimated by the dye binding capacity of the albumin, usually using 2-(4’hydroxyazobenzene) (HABA). However, it has been shown that a variety of drugs will upset dye uptake by the albumin (1). In addition, the absorbance of the native serum is so high, and th...

Serum immune globulins in Sjögren's syndrome.

Sj6gren's syndrome, a disease principally affecting exocrine glands but with systemic manifestations, is characterized by hypergammaglobulinaemia and the presence of a wide variety of autoantibodies. These are of several types, both organ and non-organ specific, complement-fixing, and precipitating antibodies, and in general are found in a higher proportion of patients than, for instance, in sy...